Facilities
Genomics
The Genomics Core supports research with DNA/RNA sequencing, genotyping, and expression analysis, offering high-throughput, long-read, single-cell, and spatial technologies, plus training and consultation for studies of genetic disease, biomarkers, and complex traits.
About
The Bindley Bioscience Genomics core provides a range of essential services to support research in genetics and molecular biology, including DNA and RNA sequencing, genotyping, and gene expression analysis. These services are instrumental in studying genetic diseases, identifying biomarkers for diagnosis and prognosis, and understanding the genetic basis of complex traits. Researchers can access equipment and expertise to prepare libraries, perform high-throughput sequencing, long read and single cell sequencing, and spatial expression analysis. The core also offers training and consultation services in experimental design and data analysis.
In the genomics core, we understand that researchers come to us not only for access to cutting-edge technologies but also for the knowledge and support to effectively navigate complex genomic analyses. We collaborate closely with researchers at every stage of their projects, to ensure that researchers achieve robust results. This approach ensures the quality and reliability of research outcomes and empowers researchers to leverage genomic data effectively in their respective fields.
Capabilities
Library Construction and Quality Control
- DNA library construction – genome resequence, Amplicon, RADseq, native barcode
- RNA library construction – Poly A+, Ribo-depletion, cDNA, Amplicon
- Library titration
- RNA and DNA preps
DNA and RNA Sequencing
- Next generation sequencing
- Small genoWith the Oxford Nanopore GridION and PromethION, the core can analyze long fragments of DNA to resolve complex genomic regions, including structural variations and repetitive sequencesme and plasmid sequencing (WideSeq)
Long Read Sequencing
With the Oxford Nanopore GridION and PromethION, the core can analyze long fragments of DNA to resolve complex genomic regions, including structural variations and repetitive sequences
Single Cell RNA sequencing
This technique measures gene expression in individual cells, revealing cell-to-cell variations and cellular heterogeneity which may be masked by traditional RNA sequencing. It can be used to study complex tissues, and characterize different cell types and responses.
Single Cell RNA sequencing
The core is able to conduct whole transcriptome analysis of tissue sections, preserving spatial resolution and location information.
Consultation
The core provides a variety of consulting services, including assistance with assay and protocol development, as well as input for grant proposals and manuscripts
Instrumentation
For single-cell genomics
For single-cell genomics, we utilize:
- The 10X Genomics Chromium Controller.
Our sequencing capabilities include
- Element Biosciences Aviti
- Two Oxford Nanopore Technologies nanopore sequencers (gridION and promethION 2 solo).
Rates
| Instrument/Operation | Assay/Prep | Internal |
|---|---|---|
| MiSeq | Single-read 50 base read or Paired 25 base reads Reagent Kit v2 (50-cycles) | $1,459 |
| MiSeq | Paired-end 150 base reads Reagent Kit v3 (150-cycle) | $1,701 |
| MiSeq | Paired-end 250 base reads Reagent Nano Kit v2 (500-cycles) | $1,554 |
| MiSeq | Paired-end 250 base reads Reagent Kit v2 (500-cycles) | $1,839 |
| MiSeq | Paired-end 300 base reads Reagent Kit v3 (600-cycle) | $2,214 |
| MiSeq | Paired-end 150 base reads Reagent Micro Kit v2 (300-cycles) | $1,060 |
| MiSeq | Paired-end 150 base reads Reagent Nano Kit v2 (300-cycles) | $903 |
| MiSeq | Paired-end 250 base reads Reagent Nano Kit v2 (300-cycles) | $965 |
| Agilent TapeStation | DNA fragment, Genomic DNA or High or Low Concentration RNA “Tapes” in stock | $9.00 |
| qPCR | KAPA library titration | $7.00 |
| FluorQubit | Fluorimetric conc. Determination | $3.00 |
| WideSeq | Full Plasmid Sequence | $21.00 |