Facilities
Light and fluorescence microscopy
Purdue’s Imaging Facility provides advanced light microscopy, including confocal, multi-photon, and light-sheet imaging, plus pre-clinical bioluminescence, fluorescence, and CT, supporting research and development for Purdue and the broader academic and non-academic community.
About
Purdue’s imaging facility is a research resource for Purdue faculty, staff, students, and other academics and non-academics seeking access to state-of-the-art light microscopy for their research and development work. We specialize in confocal, multi-photon, and light-sheet microscopy, as well as pre-clinical bioluminescence/fluorescence and computed tomography.
We serve researchers at Purdue University and biotech companies in the community as a member of the Indiana CTSI service core community. We provide the instruments and expertise needed to visualize molecules in preparations ranging from single cells to entire animals. All facility users receive individualized instrument training and project-specific advice for optimal data acquisition. Consultation on sample preparation, image rendering, and data analysis is also available as our knowledge base permits. We encourage collaborations and contract research services.
capabilities
Confocal microscopy
Our facility provides three visible-wavelength laser point scanning confocal systems suitable for creating multi-dimensional images of living or non-living samples.
- Multi-colored fluorescence imaging
- Photoablation/photoactivation
- Imaging in aqueous environments
- Material science – fluorescence and reflectance
Multiphoton microscopy
If your sample is thicker than 100 µm or you require less phototoxicity to your living sample, you might benefit by using a multi-photon (MP)
- Multi-dimensional images of thick samples, up to 800 microns
- Live cell and live animal (intravital) imaging
- Photo-uncaging
- Photoablation/photoactivation
- Second Harmonic Generation (non-centrosomatic)
TIRF microscopy
Total Internal Reflection Fluorescence uses a finite wave-front of energy to fluorescently illuminate samples and limit excitation of fluorophores close to the coverslip to dramatically increase the signal to noise of your sample.
- Protein dynamic studies on the cell membrane
- Single molecule examinations
Super Resolution microscopy
For spatial resolution beyond the diffraction-limit (<220 nm) we offer Super Resolution microscopy with both SIM (3 colors) and STORM.
- Very high resolution intracellular imaging in thin samples
- Detailed localization analysis
Wide-field imaging
- Very high resolution intracellular imaging in thin samples
- Detailed localization analysis
Pre-clinical molecular and microCT
The Bindley Imaging Facility provides systems capable of imaging larger subjects such as small rodents, medical devices or other non-biologics
- Small Animal CT Imaging
- Material science – Computed Tomography
- Fluorescent and Bioluminescent Imaging
Image processing and analysis for all data
Images are great to display but have so much more data. Use one of our commercial analysis software tools to pull out all the information.
- Co-localization analysis
- Intensity analysis
- Time-lapse imaging of dynamic intracellular events
- Three-dimensional morphology
- Deconvolution
Our team is dedicated to helping facility users maximize the value of their imaging data. We offer:
- Training on software tools and analysis workflows
- Consultation for experimental design and data interpretation
- Custom pipeline development and scripting support for specialized or high-throughput analysis needs
Light Sheet Microscopy
Light sheet microscopy excels at capturing fast 3D imaging of living or optically cleared samples with minimal light exposure:
- Rapid volumetric imaging with minimal phototoxicity and bleaching
- Multi-view acquisition for isotropic resolution
- Compatible with live embryos, organoids, spheroids, small organisms, and cleared tissue samples
- High temporal resolution for developmental and dynamic processes
- Long-term imaging of living samples
Instrumentation: Microscopy
Instrumentation: Pre-clinical
Instrumentation: Image Analysis
All workstation computers can be accessed either in person or remotely through TeamViewer. They are free to use, but advance reservation in iLab is required to use the software.
Rates
| Service | Internal Customers | PCPB Customers | External Non-Profit & Small Business | External For Profit |
|---|---|---|---|---|
| Nikon A1RMP-HD Intravital Confocal | $54/hr | $54/hr | $108/hr | $115/hr |
| Perkin Elmer Micro-CT | $48/hr | $48/hr | $181/hr | $192/hr |
| Spectral Ami Optical Imaging System | $39/hr | $39/hr | $68/hr | $73/hr |
| MiLabs U-SPECT-II/CT/PET | $72/hr | $72/hr | $163/hr | $173/hr |
| Nikon A1R-MP | $53/hr | $37/hr | $83/hr | $88/hr |
| Nikon A1Rsi | $40/hr | $28/hr | $132/hr | $141/hr |
| Super Resolution Nikon N-STORM/N-SIM/TIRF | $52/hr | $37/hr | $82/hr | $87/hr |
| Zeiss LSM 880 | $38/hr | $29/hr | $71/hr | $76/hr |
| Zeiss LSM 880 – after hours | $28/hr | $26/hr | $44/hr | $47/hr |
| Nikon 90i Wide-Field | $0/hr | $0/hr | $0/hr | $0/hr |
| Nikon TiS Wide-Field | $0/hr | $0/hr | $0/hr | $0/hr |
| Training: Confocals, Super Resolution, VECTor/CT | $224 | $152 | $347 | $347 |
| Training: AMI, Widefield, Plate Readers | $124 | $124 | $192 | $192 |
| Synergy Neo Plate Reader | $15/hr | $15/hr | $23/hr | $24/hr |
| DynaPro II Plate Reader | $10/hr | $10/hr | $17/hr | $18/hr |