Metabolomics

This UPLC/MS/MS instrument consists of an Agilent 1290 Infinity II UPLC, coupled to an Agilent 6546 quadrupole time-of-flight tandem mass spectrometer.  This instrument offers high resolution mass accuracy over a wide dynamic range, with a mass accuracy typically less than 5 ppm.  Non-targeted (discovery) experiments can be performed.  The MS/MS capability offers improved compound identification.  Comparative analysis will be performed using Agilent’s Mass Profiler Professional bioinformatics software.

A Thermo TSQ 8000 triple quadrupole mass spectrometer is coupled with a Thermo Trace 1310 GC.  Ionization is performed using either electron impact (EI) or chemical ionization (CI).  A RSH autosampler is employed, enabling either liquid, headspace, or SPME sample analysis.  Acquisition and data analysis is performed using Chromeleon software.  Detection limits of ppb are possible.

The Agilent 6410 Triple Quad provides high-level sensitivity for selected ion monitoring and multiple reaction monitoring for biomarker quantification studies. The HPLC system offers nano and microliter flow rates.  The 6410 includes both ESI and photoionization capability and has an onboard chip cube nanoLC system. 

A stand-alone Agilent 1100 HPLC system supports both ultraviolet and fluorescence detection. The HPLC pump is a binary system capable of up to a 10 ml/min flow rate and 400 bar of pressure. The UV-vis detector is a diode array, with a wavelength range from 190-950 nm. The fluorescence detector provides simultaneous quantitative and qualitative information for up to four signals simultaneously. The system also incorporates a mobile phase degasser, column heater and 100 vial autosampler. Data acquisition and analysis is performed using ChemStation software.  

The Agilent 6460 triple quadrupole mass spectrometer coupled with the Agilent 1200 Rapid Resolution HPLC provides highest sensitivity for small molecule profiling and MS/MS level analyses. This instrument provides extremely high sensitivity and throughput for small molecule analysis. Two such instruments are available.

The Waters SYNAPT XS High Definition Mass Spectrometry system is a unique, versatile platform that provides high-resolution, ion mobility-enabled, quadrupole time-of-flight (QToF) mass spectrometry. The SYNAPT is equipped with matrix-assisted laser desorption ionization (MALDI) and desorption electrospray ionization (DESI) sources for mass spectrometry imaging, which allows visualization of the spatial distribution of small molecules (metabolites, lipids), peptides and proteins in biological samples. Beyond imaging experiments, the SYNAPT can also be used for ultra-performance liquid chromatography-ion mobility-mass spectrometry (UPLC-IM-MS).

MALDI-ToF for the analysis of intact, non-volatile compounds up to 100,000 m/z.

MALDI-ToF/ToF instrument with MS/MS capability for compound characterization and identification.

Gas chromatograph coupled to a quadrupole mass spectrometer. Utilizes an electron impact (EI) and chemical ionization (CI) probe. For analysis of volatile compounds up to 1000 m/z.

HPLC system coupled to a Q-ToF mass spectrometer. Utilizes ion funnel technology for increased sensitivity and reduced limit of detection. MS/MS and MRM capabilities along with high resolution (50,000) and accurate mass (< 5 ppm) detection. Mass range up to 10,000 m/z.

Walk-up instrument located in DRUG 140A. Only available to users with card swipe access to DRUG. Single quadrupole instrument with electrospray ionization. Also capable of direct analysis of TLC plates. APCI ionization available upon request. For compounds up to 2000 m/z. To obtain access to this instrument, please submit a training request through iLab.

Automated liquid handling compatible with standard 96- or 384-well plates, as well as an assortment of custom and commercial reservoirs. Heating blocks (96-well, glass vial inserts, capability for 4 parallel plate incubation) are available for high temperature incubation. Standard plate incubation/shaker is also available. 

Linear ion trap capable of MS and MSⁿ experiments with typically unit resolution and within a 50 to 2,000 m/z range. Instrument is coupled with a Prosolia 2D desorption electrospray ionization (DESI) stage for automated high throughput analysis.

Quadrupole time-of-flight capable of MS, MS² and ion mobility experiments with >20,000 FWHM resolution and within a 20 and 100,000 m/z range.  The instrument is coupled with a 2D desorption electrospray ionization (DESI) stage equipped with a XS generation DESI sprayer and a heated transfer capillary.