Light and fluorescence microscopy

• Inverted microscope (Nikon TiE based)
• Motorized XY stage 
• Piezo z-drive and standard TiE z-motor 
• Laser: 445 nm, Argon: (457 nm, 476 nm, 488 nm, 514 nm), 561 nm, 640 nm 
• Four different PMT detectors or 32-channel spectral detector 
• Transmitted detector for DIC-like confocal imaging 
• Reflectance imaging 
• Hybrid scanner (galvanometer and resonant – up to 30 fps) 
• Live imaging with Tokai Hit stage-top chamber 
• Perfect focus system keep area of interest in focus 
• NIS-Elements software encompasses acquisition through analysis 
• FRAP and FRET experiment possible 
• Nikon Objective Choices

For additional detailed specifications, please contact the facility manager.

Location: Bindley Bioscience Center

• Inverted micrscope (Nikon TiE based) 
• Motorized XY stage 
• Standard TiE Z-motor 
• 405 nm, Argon: (457 nm, 476 nm, 488 nm, 514 nm), 561 nm, 640 nm 
• Tunable Spectra Physics Mai Tai DeepSee Ti:Sapphire laser (690-1040nm) for Multi-Photon applications and stimulation 
• Four different PMT detectors 
• Transmitted detector for DIC-like confocal imaging 
• Hybrid scanner (galvanometer and resonant – up to 30 fps) 
• Live imaging with Tokai Hit stage-top chamber (inquire about dish compatibility) 
• NIS-Elements software encompasses acquisition through analysis 
• Nikon Objective Choices 

For additional detailed specifications, please contact the facility manager.

Location: Bindley Bioscience Center

• Upright microscope (Nikon FN-1)
• Dual Coherent IR lasers (tuneable 800 – 1300 nm and fixed 1040 nm) for multi-photon imaging 
• Three GaAsP and one high-sensitive red non-descanned detectors 
• Second Harmonic Generation for imaging collagen 
• Anesthesia equipment and heat plate for animal imaging 
• Visible lasers 405 nm, 488 nm, 561 nm, 640 nm for standard or optogenetics imaging 
• Spectral GaAsP Detector for standard visible confocal 
• Hybrid scanner (galvanometer and resonant – up to 30 fps) 
• Motorized Prior XYZ 
• High-speed piezo-Z motor 
• Prior Scientific Z-Deck motorized stage 
• Nikon Objective Choices 

For additional detailed specifications, please contact the facility manager.

Location: Bindley Bioscience Center

• Inverted microscope (Nikon TiE based) 
• Motorized stage 
• Piezo z-drive 
• Perfect focus system 
• STORM (or cSTORM) with 647nm, 561nm and 488nm lasers 
• SIM with 405nm, 488nm and 561nm lasers 
• TIRF capability (multicolor TIRF (10 ms/channel)) 
• Nikon Objective Choices 

For additional detailed specifications, please contact the facility manager.

Location: Bindley Bioscience Center

• Upright microscope (Nikon 90i based) 
• Differential Interference Contrast (DIC) and standard polarizers 
• Brightfield 
• Nikon DS-Ri1 12MP color camera 
• Manual stitching with NIS-Elements Software 
• Nikon Objective Choices 

For additional detailed specifications, please contact the facility manager.

Location: Bindley Bioscience Center

• Inverted microscope (Nikon TiS) 
• Phase contrast and brightfield 
• Zenon light source for fluorescence imaging 
• High sensitive Photometrics QuantEM EMCCD camera 
• Standard fluorescent filter cubes including unique far-red filters 

• Nikon Objective Choices 

For additional detailed specifications, please contact the facility manager.

Location: Bindley Bioscience Center Room 233

• Upright microscope (Zeiss Axio Examiner Z1) 
• Laser: 405 nm, Argon: (458 nm, 488 nm, 514 nm), 561 nm and 633 nm. 
• Detectors: 
  • 32-channel GaAsP spectral photomultiplier tube (PMT) 
  • 2 MA-PMT for blue and red channels 
  • Transmitted light PMT 
• Unique objective turret for both standard and dipping applications 

For additional detailed specifications, please contact the facility manager.

Location: Hansen Lifescience Building

The lightsheet system is perfect for capturing 3D fluorescent images of organisms, tissue explants, and cell cultures over long periods. Basically, it shines a thin sheet of laser light on a slice of your sample, and high-speed cameras take pictures of the glowing parts from the side. Using the light sheet method means less damage to your sample from laser exposure, which is great for imaging delicate things like embryos, larvae, and organoids. You can use it to image alive, preserved, or optically cleared samples.

• Two Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lenses for illumination. 
• Two Olympus XLUMPLFLN 20x (eff. 22.2x) W 1.0 NA water immersion objective lenses for detection 
• Two high-speed Hamamatsu Orca Flash 4.0 V3 sCMOS cameras 
• Laser wavelength: 405 nm, 488 nm, 515 nm, 561 nm, 642 nm, 785 nm 
• Flexible light-sheet thickness (2 µm to 4 µm) 
• Filters: 
  • Bandpass 418-462, 457-501, 500-530, 526-564, 580-627, 655-704 
  • Longpass 498 
• Photomanipulation module: 532 nm ablation 

For additional detailed specifications and sample preparation, please contact the facility manager.

Location: Hansen Lifescience Building B032

The Ami can perform either Fluorescence or Bioluminescent Imaging with a standard photography overlay.

• 25 x 17 cm to 10 x 7 cm Field of View 
• Imaging Platform 56 x 66 x 122 cm3 (width x depth x height) 
• Heated imaging platform 
• Free vendor software available for download 

(All values ± 10 nm range)

For additional detailed specifications, please contact the facility manager.

Location: Hansen Lifescience Building

• 72 mm Field of View 
• Down to 4.5 µm resolution after sub or slice reconstruction 
• Max. tube voltage 90 kV 
• Max. tube current 200 µA 
• Max. output 8 W 
• 60 fps 
• Invivo imaging capability for different animals 

For additional detailed specifications, please contact the facility manager.

Location: Hansen Lifescience Building

Nuclear medicine pre-clinical instrument for PET/SPECT/CT.

• Resolution 0.35 – 0.8 mm based upon collimator 
• Multi-isotope imaging (20 keV – 511 keV) 
• Auto-fusion of CT images 
• Interchangeable and easy-to-mount collimators 

For additional detailed specifications, please contact the facility manager.

Location: Hansen Lifescience Building